R&D Introduction

①　Clostridium butyricum powder

②　 Functional compound probiotics dominated by Clostridium butyricum

③　Clostridium butyricum derivatives: including anti-biotics (probiotics + fermentation natural products) : Combo (piglets/eggs/aquatic products), Yiqikang, Yishengtai, etc.

Scientific formula: by comparing the number of bacteria in different depths of water, lactic acid bacteria, bacillus, yeast, nitrifying bacteria and other active bacteria are matched in accordance with the appropriate proportion, and the proportion is scientific to better regulate the water quality.

Quick results: select bacteria with strong activity and quick activation, rich metabolites and strong acid production ability, which can quickly regulate water quality.

Easy to use: water can be directly splashed

Improve AT and prolong the time of changing water : by metabolizing organic matter in water by beneficial bacteria, increase AT value in water and prolong the time of changing water by 2-3 times.

Site of action, mechanism of acid production, metabolites

First of all, the content of butyrate in triglyceride is small, and the actual use of 2-3 kg/ton has no obvious effect. In addition, the effect of butyrate and sodium butyrate depends on the coating process, and the coating capacity determines whether they can be transported to the hindgut to play a role. In addition, compared with Clostridium butyricum, the effect of the former two is one-time supply, while Clostridium butyricum is living bacteria can increase in value, and the effect is continuous.

Clostridium butyricum is resistant to some antibiotics such as cephalosporin cefepime ammonia benzyl, cefazolin, quinolone norfloxacin, sulfa new connaught Ming, aminoglycoside compound gentamycin, tetracycline, streptomycin B and so on. But it’ s meaningless despite dose toxicity. Therefore the use of Clostridium butyricum is recommended after the end of the antibiotic metabolic cycle.

Bacillus ---- is commonly used in silage but does not survive for long

Spore ---- is aerobic and produces enzymes to help digestion in the small intestine, but the biological oxygen is consumed at the same time, adding too much will lead to the animal "only eat not long".

Saccharomyces cerevisiae ---- had good food lure effect and enzyme production performance.

Clostridium butyricum ----1. Does not compete with the host for nutrition; 2.The hindgut plays a role of secondary fermentation, conducive to nutrient absorption; 3. Butyric acid production, a variety of physiological effects (such as: repair intestinal mucosal barrier, reduce muscle lactic acid accumulation)

In terms of reducing FCR, clostridium butyricum has a better effect than bacillus. Compared with foregut, hindgut has a greater effect on digestion and energy absorption of piglets, and hindgut "secondary digestion" can not be digested and absorbed by small intestine. For example, oligosaccharides are digested and utilized under the action of hindgut microbes, while Clostridium butyricum mainly acts in the hindgut. In addition, compared with aerobic bacteria, Clostridium butyricum does not need oxygen for proliferation and consumes less energy, so it has a better effect in reducing FCR.

Environmental stress (high density farming, pathogenic bacteria, etc.) continues to stimulate, so after adding Clostridium butyricum, the use of clostridium butyricum should continue for 7-14 days until the intestinal tract reaches a relatively stable and healthy state. The better the environment and gut health, the less clostridium butyricum is needed.

Intestinal tract and systemic many viscera can have " communication ", the " language " of communication is intestinal tract microorganism, way basically has two: nervous system and blood circulation system. As intestinal probiotics, Clostridium butyricum colonizes in the hindgut and can enter the blood circulation through its metabolites. As blood participates in the regulation of the whole body.

Clostridium butyricum is a microorganism with strong stress resistance. In actual production, it was found that the strain could still grow well when pH reached 11. In terms of acid resistance, it was found that spores did not change until pH was from 1.3 to 1.4, with a decrease of about 6%. The normal PH value of the gastrointestinal tract of pigs is 3-4, and the starvation state will reach 1-3. Our experiment proved that the strain will change greatly only when the PH value is less than 1.3, so there is no problem in using it on pigs.

Clostridium butyricum slowly transforms from vegetative to spore. We have also made some attempts on lactic acid. We will do post-processing in the propagation stage and plan to do it in the logarithmic growth stage to see whether the spore rate reaches the highest.

Biosafety is the lifeblood of microbial enterprises also is our primary problem to eliminate, so far, there has been no pollution problem in the laboratory, mainly lies in the process of control, whether in the fermentation process of air, pipe or laboratory disinfection and sterilization have very strict requirements. In addition, our company implements the project team system, and each person is responsible for one strain of bacteria, which reduces the possibility of cross contamination.

Most Clostridium butyricum of acid production, enzyme production and other characteristics have little difference, the main lies in the shelf life, in other words, sporulation rate. Different of other manufacturers, JBH’Clostridium butyricum is tested for spore rate, which is used to evaluate the final quality of products, while many products on the market are based on live bacteria number. According to the test data of each batch of products, the spore transfer rate of our clostridium butyricum products is almost 100%. Another look from the storage time and conditions, from the raw material transport to swim under cover factory made into finished products, and delivery to the dealership network at all levels on the market, this process will take at least more than a month of time, will stay in the warehouse after two to three months, or even more than six months time, also affected by the transport and storage conditions, and our products on the surrounding environment is tolerance, The shelf life is also relatively long.

Clostridium butyricum adheres and colonizes the hindgut. Compared with the foregut, the hindgut has a greater effect on digestion and absorption of energy. The hindgut's secondary digestion can digest and absorb which the small intestine can't. For example, oligosaccharides are decomposed, digested and utilized under the action of the hindgut microorganisms, while the main action site of Clostridium butyricum is the hindgut. In addition, compared with aerobic bacteria, the growth of Clostridium butyricum does not require oxygen and consumes less energy. So Clostridium butyricum can significantly promote FCR.

Clostridium butyricum is more conducive to intestinal health. Clostridium butyricum is a butyric acid-producing bacteria. The importance of butyric acid-producing bacteria lies in two aspects: On the one hand, they themselves promote the body's intestinal health in a variety of ways. On the other hand, the "prebiotic" effect of some probiotics such as lactic acid bacteria can only be achieved by butyric acid-producing bacteria, so butyric acid-producing bacteria are more conducive to intestinal health.